EphA3 Antibody - C-terminal region (OAAB17128)

Catalog No: OAAB17128

Size:400ul

Western blot analysis of anti-EphA3 Pab (Cat. # OAAB17128) in CHO cell lysate. EphA3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

There is BioGPS gene expression data showing that EPHA3 is expressed in HEK293

Western blot analysis of EphA3(arrow) using rabbit polyclonal EphA3 Antibody (C-term) (Cat. # OAAB17128).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human placenta tissue reacted with EphA3 antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: EphA3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development
  Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein
  Tissue Location: Widely expressed. Highest level in placenta.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This EphA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-928 amino acids from the C-terminal region of human EphA3.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that EPHA3 is expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: EPHA3
• Alias Symbols: EK4, ETK, HEK, ETK1, HEK4, TYRO4
• NCBI Gene Id: 2042
• Protein Name: Ephrin type-A receptor 3
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P29320
• Protein Accession #: NP_005224.2
• Molecular Weight: 110131 Da