EPHA3 Antibody (Bi-Phospho-Tyr779/833) (OAAF00439)

Catalog No: OAAF00439 (Formerly GWB-ASB883)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain, using EPHA3/4/5 (Phospho-Tyr779/833) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human EPHA3/4/5 around the phosphorylation site of Tyr779/833.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: EPHA3/4/5 (Phospho-Tyr779/833) antibody detects endogenous levels of EPHA3/4/5 only when phosphorylated at tyrosine 779.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:10000
• Reference: Wicks I.P., Proc. Natl. Acad. Sci. U.S.A. 89:1611-1615(1992).
  Chiari R., Cancer Res. 60:4855-4863(2000).
  Boyd A.W., J. Biol. Chem. 267:3262-3267(1992).

Target Info

• Gene Symbol: EPHA3|EPHA4|EPHA5
• Gene Full Name: EPH receptor A3|EPH receptor A4|EPH receptor A5
• Alias Symbols: brain-specific kinase;CEK7;EHK1;EHK-1;EK4;EK7;EK8;EPH homology kinase 1;EPH-like kinase 4;EPH-like kinase 7;EPH-like kinase 8;eph-like tyrosine kinase 1;ephrin type-A receptor 3;ephrin type-A receptor 4;ephrin type-A receptor 5;epididymis secretory sperm binding protein;ETK;ETK1;HEK;HEK4;HEK7;HEK8;human embryo kinase 1;receptor protein-tyrosine kinase HEK7;receptor protein-tyrosine kinase HEK8;SEK;testicular tissue protein Li 64;TYRO1;TYRO1 protein tyrosine kinase;TYRO4;TYRO4 protein tyrosine kinase;tyrosine-protein kinase receptor EHK-1;tyrosine-protein kinase receptor ETK1;tyrosine-protein kinase receptor SEK;tyrosine-protein kinase TYRO1;Tyrosine-protein kinase TYRO4.
• NCBI Gene Id: 2042|2043|2044
• Protein Name: Ephrin type-A receptor 3|Ephrin type-A receptor 4|Ephrin type-A receptor 5
• Description of Target: Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.|Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).|Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
• Uniprot ID: P29320|P54756|P54764
• Protein Accession #: NP_005224.2
• Molecular Weight: 110 kDa