EPHA1 ELISA Kit (Human) (OKCD01580)

Catalog No: OKCD01580 (Formerly GWB-KBBOR1)

Size:96WELLS

Human EPHA1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type A Receptor 1 (EPHA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin Type A Receptor 1 (EPHA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type A Receptor 1 (EPHA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ephrin Type A Receptor 1 (EPHA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin Type A Receptor 1 (EPHA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin Type A Receptor 1 (EPHA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EPHA1 Microplate: 96 Wells (12 x 8 Well Strips)
  • EPHA1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated EPHA1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ephrin Type A Receptor 1 (EPHA1). No significant cross-reactivity or interference between Ephrin Type A Receptor 1 (EPHA1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: EPHA1
• Gene Full Name: EPH receptor A1
• Alias Symbols: EPH;EPH tyrosine kinase;eph tyrosine kinase 1;ephrin type-A receptor 1;EPHT;EPHT1;erythropoietin-producing hepatoma amplified sequence;erythropoietin-producing hepatoma receptor;hEpha1;oncogene EPH;soluble EPHA1 variant 1;soluble EPHA1 variant 2;tyrosine-protein kinase receptor EPH.
• NCBI Gene Id: 2041
• Protein Name: Ephrin type-A receptor 1
• Description of Target: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
• Uniprot ID: P21709
• Protein Accession #: NP_005223.4
• Nucleotide Accession #: NM_005232.4
• Protein Size (# AA): 976