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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for EPAS1 Antibody (OACD04185) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | Range: Arg24 - Glu348 |
| Purification | Affinity Purified; Protein A Purified |
| Concentration | 0.5 mg/ml |
| Preservative | 0.02% NaN3 |
| Specificity | The antibody is a rabbit polyclonal antibody raised against HIF2a. It has been selected for its ability to recognize HIF2a in immunohistochemical staining and western blotting. |
| Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
| Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
| Gene Symbol | Epas1 |
|---|---|
| Gene Full Name | endothelial PAS domain protein 1 |
| Alias Symbols | endothelial PAS domain-containing protein 1;EPAS-1;HIF2 alpha;HIF-2 alpha;Hif2a;HIF2-alpha;HIF-2-alpha;HLF;hypoxia inducible factor 2, alpha subunit;hypoxia inducible factor 2a;hypoxia-inducible factor 2-alpha. |
| NCBI Gene Id | 29452 |
| Protein Name | Endothelial PAS domain-containing protein 1 |
| Description of Target | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). |
| Uniprot ID | Q9JHS1 |
| Protein Accession # | NP_075578.1 |
| Nucleotide Accession # | NM_023090 |
| Molecular Weight | 97 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EPAS1 Antibody (OACD04185)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "EPAS1 Antibody (OACD04185)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "EPAS1 Antibody (OACD04185)" provided in?
This item is provided in "Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EPAS1 Antibody (OACD04185)"?
This target may also be called "endothelial PAS domain-containing protein 1;EPAS-1;HIF2 alpha;HIF-2 alpha;Hif2a;HIF2-alpha;HIF-2-alpha;HLF;hypoxia inducible factor 2, alpha subunit;hypoxia inducible factor 2a;hypoxia-inducible factor 2-alpha." in publications.
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What is the shipping cost for "EPAS1 Antibody (OACD04185)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EPAS1 Antibody (OACD04185)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EPAS1 Antibody (OACD04185)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "97 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EPAS1 Antibody (OACD04185)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Epas1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Epas1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Epas1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Epas1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Epas1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Epas1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
