EPAS1 Antibody - middle region (ARP32253_P050)

Catalog No: ARP32253_P050

WB Suggested Anti-EPAS1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EPAS1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: ESLFKPHLMAMNSIFDSSGKGAVSEKSNFLFTKLKEEPEELAQLAPTPGD
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-EPAS1 (ARP32253_P050) antibody is Catalog # AAP32253 (Previous Catalog # AAPP03234)
• Reference: Koizume,S., (2008) Biochem. Biophys. Res. Commun. 371 (2), 251-255

Target Info

• Gene Symbol: EPAS1
• Gene Full Name: Endothelial PAS domain protein 1
• Alias Symbols: HLF, MOP2, ECYT4, HIF2A, PASD2, bHLHe73
• NCBI Gene Id: 2034
• Protein Name: Endothelial PAS domain-containing protein 1
• Description of Target: EPAS1 is a transcription factor involved in the induction of oxygen regulated genes. EPAS1 binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. EPAS1 regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. EPAS1 may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. EPAS1 is a potent activator of the Tie-2 tyrosine kinase expression. The activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction of EPAS1 with redox regulatory protein APEX seems to activate CTAD.
• Uniprot ID: Q99814
• Protein Accession #: NP_001421
• Nucleotide Accession #: NM_001430
• Protein Size (# AA): 870
• Molecular Weight: 90kDa
• Protein Interactions: MAP1LC3A; SQSTM1; LAMP1; BATF2; BATF3; BATF; DDIT3; DBP; NDN; JUN; EPAS1; NPM1; BBS4; BBS2; BBS1; Arnt; VHL; STAT5A; SMAD3; EWSR1; MED11; MED8; EGLN3; PSMD1; PSMB1; MED1; PHB; MITF; HIF1A; EP300; EMD; CTNNB1; CREBBP; EGLN2; DPY30; MED10; MED25; ARID1B; WR