ABCG2 ELISA Kit (Pig) (OKEH06613)

Catalog No: OKEH06613

Size:96T

Pig ABCG2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Abcg2 ELISA Kit (Pig) (OKEH06613) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abcg2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Abcg2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abcg2 captured in the well.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • Abcg2 Microplate: 96 Wells (12 x 8 Well strips)
  • Abcg2 Lyophilized Standard: 2 x 20 ng
  • 100X Abcg2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.101 ng/mL
• Specificity: Natural and recombinant Pig ATP-binding cassette sub-family G member 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ABCG2
• Gene Full Name: ATP-binding cassette, sub-family G (WHITE), member 2
• Alias Symbols: ATP-binding cassette sub-family G member 2;BMDP;brain multidrug resistance protein;broad substrate specificity ATP-binding cassette transporter ABCG2;urate exporter.
• NCBI Gene Id: 397073
• Protein Name: Q8MIB3
• Description of Target: ATP-binding cassette sub-family G member 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q8MIB3
• Protein Accession #: NP_999175
• Nucleotide Accession #: NM_214010