Catalog No: OKCD07155
Size:96WELLS
Price: $750.00
SKU
OKCD07155
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for ENTPD1 ELISA Kit (Human) (OKCD07155) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.062ng/mL |
Gene Symbol | ENTPD1 |
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Gene Full Name | ectonucleoside triphosphate diphosphohydrolase 1 |
Alias Symbols | ATPDase;CD39;CD39 antigen;ecto-apyrase;ecto-ATP diphosphohydrolase 1;ecto-ATPase 1;ecto-ATPDase 1;ectonucleoside triphosphate diphosphohydrolase 1;lymphoid cell activation antigen;NTPDase-1;SPG64. |
NCBI Gene Id | 953 |
Protein Name | Ectonucleoside triphosphate diphosphohydrolase 1 |
Description of Target | In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. |
Uniprot ID | P49961 |
Protein Accession # | NP_001091645.1 |
Nucleotide Accession # | NM_001098175.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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