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Catalog No: OKEH06955
Size:96T
Price: $625.00
SKU
OKEH06955
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Enpp2 ELISA Kit (Rat) (OKEH06955) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Enpp2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Enpp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Enpp2 captured in the well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <4.9%
Inter-Assay CV <8.2%
ELISA Kit Component
ComponentAmount
Enpp2 Microplate96 Wells (12 x 8 Well strips)
Enpp2 Lyophilized Standard2 x 40 ng
100X Enpp2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.316 ng/mL
SpecificityNatural and recombinant Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEnpp2
Gene Full Nameectonucleotide pyrophosphatase/phosphodiesterase 2
Alias Symbolsautotaxin;ectonucleotide pyrophosphatase/phosphodiesterase family member 2;E-NPP 2;extracellular lysophospholipase D;lysoPLD;phosphodiesterase I/nucleotide pyrophosphatase 2 (autotaxin).
NCBI Gene Id84050
Protein NameQ64610
Description of TargetEctonucleotide pyrophosphatase/phosphodiesterase family member 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q64610
Protein Accession #NP_476445
Nucleotide Accession #NM_057104
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