ENPP1 ELISA Kit (Mouse) (OKCA02376)

Catalog No: OKCA02376 (Formerly GWB-Y6QTK3)

Size:96 Wells

Mouse ENPP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  1:5	93	89-97
  1:10	90	82-99
  1:20	95	87-101
  1:40	95	85-104

• ELISA Kit Principle: Aviva Systems Biology Enpp1 ELISA Kit (Mouse) (OKCA02376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Enpp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Enpp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Enpp1 captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	95	88-99
  EDTA Plasma (n=4)	95	90-100

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Enpp1 Microplate: 96 Wells (12 x 8 Well strips)
  • Enpp1 Lyophilized Standard: 2
  • 100X Biotinylated Enpp1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ENPP1
• Alias Symbols: N, P, Pd, E-N, PC-, Pca, ttw, twy, Ly-4, M6S1, NPP1, Npps, PC-1, Ly-41, Pca-1, Pdnp1, C76301, CD203c, E-NPP1, E-NPP 1, AI428932, 4833416E15Rik
• NCBI Gene Id: 18605
• Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Description of Target: Appears to modulate insulin sensitivity. By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling.
• Uniprot ID: P06802
• Protein Accession #: NP_001295256
• Nucleotide Accession #: NM_001308327