20% off all ELISAs • Two for the price of one on select antibodies.

Don’t miss out on these great offers! Learn More Here.

Catalog No: OKEH00718 (Formerly GWB-WSY3C2)
Price: $600.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
Please contact customer service when ordering 10+ kits.

Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

ENPP1 ELISA Kit (Human) : 96 Wells (OKEH00718)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00718
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ENPP1 ELISA Kit (Human) (OKEH00718) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ENPP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ENPP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ENPP1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.9% (n = 20)
Mean Inter-assay CV%: < 11% (n = 20)
ELISA Kit Component
Anti-ENPP1 Microplate96 Wells (12 x 8 Well strips)
ENPP1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ENPP1 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.04 ng/mL
SpecificityNatural and recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolENPP1
Gene Full Nameectonucleotide pyrophosphatase/phosphodiesterase 1
Alias Symbolsalkaline phosphodiesterase 1;ARHR2;COLED;ectonucleotide pyrophosphatase/phosphodiesterase family member 1;E-NPP 1;Ly-41 antigen;M6S1;Membrane component chromosome 6 surface marker 1;membrane component, chromosome 6, surface marker 1;NPP1;NPPS;PC-1;PCA1;PDNP1;phosphodiesterase I/nucleotide pyrophosphatase 1;plasma-cell membrane glycoprotein 1;plasma-cell membrane glycoprotein PC-1.
NCBI Gene Id5167
Protein NameP22413
Description of TargetEctonucleotide pyrophosphatase/phosphodiesterase family member 1
Uniprot ID
Protein Accession #NP_006199.2
Nucleotide Accession #NM_006208.2
Protein Size (# AA)925
Molecular Weight104 kDa
Write Your Own Review
You're reviewing:ENPP1 ELISA Kit (Human) : 96 Wells (OKEH00718)
Your Rating
We found other products you might like!