ENO3 Antibody (OAGA02213)

Catalog No: OAGA02213

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
OAGA02213 diluted at 1:10000

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ENO3 (OAGA02213) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ENO3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:5000-1:20000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: ENO3
• Gene Full Name: enolase 3 (beta, muscle)
• Alias Symbols: MSE, GSD13
• NCBI Gene Id: 2027
• Description of Target: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
• Uniprot ID: P13929