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ENO2 ELISA Kit (Human) : 96 Wells (OKCA00125)

Catalog#: OKCA00125
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration 2.5 Hours
Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Human Serum (n=4)1:188%84-92%
Human Serum (n=4)1:294%90-98%
Human Serum (n=4)1:499%95-104%
Human Serum (n=4)1:8102%96-108%
Kit Principle Aviva Systems Biology ENO2 ELISA Kit (Human) (OKCA00125) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human ENO2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human ENO2 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human ENO2 captured in well.
Kit Range 0.78 ng/mL - 50 ng/mL
Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n=5)92%88-96%
Sample 2
Sample TypeAverage RecoveryRange
EDTA Plasma (n=4)96%90-102%
Kit Reproducibility Intra-assay: CV% < 8% (n = 20).Inter-assay: CV% < 10% (n = 20)
Kit Component
Anti-ENO2 Microplate96 Well (12 x 8 Well Strips)
ENO2 Standard (Lyophilized)2 x 50 ng
100X Biotinylated Anti-ENO2Detector Antibody1 x 120 uL
100X HRP-Avidin1 x 120 uL
Detection Antibody Diluent1 x 15 mL
HRP-Avidin Diluent1 x 15 mL
Sample Diluent1 x 50 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates
Sensitivity < 0.195 ng/ml
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of human
NSE. No significant cross-reactivity or interference between human NSE and
analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between human NSE and all the analogues,
therefore, cross reaction may still exist.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Gene Symbol ENO2
Official Gene Full Name Enolase 2 (gamma, neuronal)
Alias Symbols 2-phospho-D-glycerate hydro-lyase, Enolase 2, Gamma-enolase, Neural enolase, Neuron-specific enolase, NSE
NCBI Gene Id 2026
Protein Name Gamma-enolase
Description of Target This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Swissprot Id P09104, Q96J33, A8K3B0
Protein Accession # NP_001966
Nucleotide Accession # NM_001975
Protein Size (# AA) 434
Molecular Weight 47 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ENO2.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ENO2.
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