ENO1 ELISA Kit (Mouse) (OKCD07404)

Catalog No: OKCD07404

Size:96WELLS

ENO1 ELISA Kit (Mouse) (OKCD07404) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enolase 1 (ENO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enolase 1 (ENO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enolase 1 (ENO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Enolase 1 (ENO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enolase 1 (ENO1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enolase 1 (ENO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ENO1 Microplate: 96 Wells (12 x 8 Well strips)
  • ENO1 Lyophilized Standard: 2
  • 100X ENO1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 31pg/mL

Target Info

• Gene Symbol: Eno1|Eno1b
• Gene Full Name: enolase 1, alpha non-neuron|enolase 1B, retrotransposed
• Alias Symbols: 2-phospho-D-glycerate hydrolase;2-phospho-D-glycerate hydro-lyase;AL022784;alpha-enolase;c-Myc promoter binding protein;EG433182;Eno;Eno1;Eno-1;Enolase 1;enolase 1, alpha non-neuron;enolase 1B, retrotransposed;Gm5506;MBP-1;NNE;non-neural enolase.
• NCBI Gene Id: 13806|433182
• Protein Name: Alpha-enolase
• Description of Target: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses (By similarity). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production (By similarity).
• Uniprot ID: P17182
• Protein Accession #: NP_001020559.1
• Nucleotide Accession #: NM_001025388.1