ENO1 Chemi-Luminescent ELISA Kit (Human) (OKCD03831)

Catalog No: OKCD03831

Size:96WELLS

Human ENO1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-92%	91-99%	97-105%	79-102%
  EDTA Plasma (n=5)	83-103%	87-94%	81-103%	93-101%
  Heparin Plasma (n=5)	94-102%	93-103%	78-103%	80-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Enolase 1 (ENO1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Enolase 1 (ENO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Enolase 1 (ENO1) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-101	97
  EDTA Plasma (n=5)	78-102	90
  Heparin Plasma (n=5)	86-101	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enolase 1 (ENO1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enolase 1 (ENO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ENO1 Microplate: 96 Wells (12 x 8 Well strips)
  • ENO1 Lyophilized Standard: 2
  • 100X ENO1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 27.4 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ENO1
• Gene Full Name: enolase 1
• Alias Symbols: 2-phospho-D-glycerate hydro-lyase;alpha enolase like 1;alpha-enolase;C-myc promoter-binding protein;c-myc promoter-binding protein-1;ENO1L1;Enolase 1;enolase 1, (alpha);enolase-alpha;epididymis secretory protein Li 17;HEL-S-17;MBP-1;MPB1;MPB-1;MYC promoter-binding protein 1;NNE;non-neural enolase;phosphopyruvate hydratase;plasminogen-binding protein;PPH;tau-crystallin.
• NCBI Gene Id: 2023
• Protein Name: Alpha-enolase
• Description of Target: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
• Uniprot ID: P06733
• Protein Accession #: NP_001188412
• Nucleotide Accession #: NM_001201483