ENO1 Antibody - C-terminal region : Biotin (ARP34376_T100-Biotin)

Catalog No: ARP34376_T100-Biotin

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Biotin
• Application: IHC, WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ENO1
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Sheep: 93%; Yeast: 79%; Zebrafish: 79%
• Peptide Sequence: Synthetic peptide located within the following region: VVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ENO1 (ARP34376_T100-Biotin) antibody is Catalog # AAP34376 (Previous Catalog # AAPY00218)
• Sample Type Confirmation:

  ENO1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Reference: Ghosh,A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330
• Publications:

  Rambaruth, N. D. S., Greenwell, P. & Dwek, M. V. The lectin Helix pomatia agglutinin recognizes O-GlcNAc containing glycoproteins in human breast cancer. Glycobiology 22, 839-48 (2012). IP, Human, Guinea pig, Horse, Rabbit, Rat, Sheep, Bovine, Dog, Mouse, Yeast, Zebrafish 22322011

Target Info

• Gene Symbol: ENO1
• Gene Full Name: Enolase 1, (alpha)
• Alias Symbols: NNE, PPH, MPB1, ENO1L1, HEL-S-17
• NCBI Gene Id: 2023
• Protein Name: Alpha-enolase
• Description of Target: ENO1 is one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
• Uniprot ID: Q53HR3
• Protein Accession #: NP_001419
• Nucleotide Accession #: NM_001428
• Protein Size (# AA): 434
• Molecular Weight: 47kDa
• Protein Interactions: UBC; FUS; HUWE1; ISG15; SUMO2; SUMO3; IVNS1ABP; STAU1; MDM2; WWOX; EED; FBXO6; UPF2; YWHAQ; UBD; TERT; SRC; RAD52; NPM1; BHLHE40; VCAM1; HCVgp1; ITGA4; FN1; ATF2; SERPING1; AMBP; LIG4; BRCA1; MDC1; PGK1; ITPA; GAPDH; FYN; FLNC; ENO3; Htt; TPI1; PPIA; AKT1