Catalog No: OKCD02078 (Formerly GWB-KBBOM1)
Size:96WELLS
Price: $750.00
SKU
OKCD02078
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for ENGASE ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endo Beta-N-Acetylglucosaminidase (ENGASE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endo Beta-N-Acetylglucosaminidase (ENGASE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Endo Beta-N-Acetylglucosaminidase (ENGASE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endo Beta-N-Acetylglucosaminidase (ENGASE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endo Beta-N-Acetylglucosaminidase (ENGASE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ENGASE Microplate96 Wells (12 x 8 Well Strips)
ENGASE Lyophilized Standard2 x 20 ng
100X Biotinylated ENGASE Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.126 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Endo Beta-N-Acetylglucosaminidase (ENGASE). No significant cross-reactivity or interference between Endo Beta-N-Acetylglucosaminidase (ENGASE) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolENGASE
Gene Full NameEndo-beta-N-acetylglucosaminidase
Alias SymbolsCytosolic endo-beta-N-acetylglucosaminidase, DKFZp434P174, ENGase, FLJ21865
NCBI Gene Id64772
Protein NameCytosolic endo-beta-N-acetylglucosaminidase
Description of TargetEndoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
Uniprot IDQ8NFI3
Protein Accession #NP_001036038.1
Nucleotide Accession #NM_001042573.2
Protein Size (# AA)743
Write Your Own Review
You're reviewing:ENGASE ELISA Kit (Human) (OKCD02078)
Your Rating
We found other products you might like!