ENG Antibody (OAEE00594)

Catalog No: OAEE00594

Size:100UG

Surface staining of CD105 in murine bone marrow with anti-CD105 (MJ7/18) PE.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MJ7/18
• Isotype: IgG2a
• Host: Rat
• Conjugation: R-PE
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Inflamed mouse skin
• Purification: Purified by size-exclusion chromatography
• Concentration: 0.5 mg/ml
• Specificity: The rat monoclonal antibody MJ7/18 reacts with CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow.
• Formulation: PE
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Dassler K, Roohi F, Lohrke J, Ide A, Remmele S, Hütter J, Pietsch H, Pison U, Schütz G: Current limitations of molecular magnetic resonance imaging for tumors as evaluated with high-relaxivity CD105-specific iron oxide nanoparticles. Invest Radiol. 2012 Jul;47(7):383-91
  Herouet C, Cottin M, LeClaire J, Enk A, Rousset F: Contact sensitizers specifically increase MHC class II expression on murine immature dendritic cells. In Vitr Mol Toxicol. 2000 Summer;13(2):113-23.
  Fonsatti E, Nicolay HJ, Altomonte M, Covre A, Maio M: Targeting cancer vasculature via endoglin/CD105: a novel antibody-based diagnostic and therapeutic strategy in solid tumours. Cardiovasc Res. 2010 Apr 1;86(1):12-9. doi: 10.1093/cvr/cvp332.
  Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64.
  Cho SK, Bourdeau A, Letarte M, Zúñiga-Pflücker JC: Expression and function of CD105 during the onset of hematopoiesis from Flk1(+) precursors. Blood. 2001 Dec 15;98(13):3635-42.
  Rosu-Myles M, She YM, Fair J, Muradia G, Mehic J, Menendez P, Prasad SS, Cyr TD: Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells. PLoS One. 2012;7(6):e38954. doi: 10.1371/journal.pone.0038954.
  

Target Info

• Gene Symbol: Eng
• Gene Full Name: endoglin
• Alias Symbols: AI528660;AI662476;CD105;cell surface MJ7/18 antigen;En;Endo;endoglin;S-endoglin;transmembrane glycoprotein.
• NCBI Gene Id: 13805
• Protein Name: Endoglin
• Description of Target: Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:10625534). Required for normal structure and integrity of adult vasculature (By similarity). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed:10625534). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (PubMed:28530658). May play a role in the binding of endothelial cells to integrins. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (By similarity).
• Uniprot ID: Q63961
• Protein Accession #: NP_000109.1; NP_001108225.1; NP_001265067.1
• Nucleotide Accession #: NM_000118.3; NM_001114753.2; NM_001278138.1