Catalog No: OKEH08606
Size:96T
Price: $725.00
SKU
OKEH08606
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)95-105% (n = 20)102-111% (n = 20)103-113% (n = 20)102-111% (n = 20)
EDTA plasma(n=5)88-100% (n = 20)95-108% (n = 20)83-96% (n = 20)110-120% (n = 20)
heparin plasma(n=5)86-96% (n = 20)84-94% (n = 20)86-96% (n = 20)86-96% (n = 20)
ELISA Kit PrincipleAviva Systems Biology ENDOU ELISA Kit (OKEH08606) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ENDOU antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ENDOU and incubated. The ENDOU found in the sample or standards competes with the biotinylated ENDOU for limited binding sites on the immobilized anti-ENDOU antibody. Excess unbound biotinylated ENDOU and sample or standard ENDOU is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ENDOU captured in the well and inversely proportional to the amount of ENDOU which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.7% (n = 20)
Mean Inter-assay CV%: <=10.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ENDOU Microplate96 Wells (12 x 8 Well strips)
ENDOU Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X ENDOU-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.
::Subcellular Location: Secreted
::Unigene: Hs.997
SMR: P21128
String: 9606.ENSP00000397679
::KEGG: hsa:8909
MIM: 606720
Pfam: PF01033
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.25ng/mL
SpecificityNatural and recombinant Human Poly(U)-specific endoribonuclease
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolENDOU
Gene Full Nameendonuclease, poly(U) specific
Alias Symbols22 serine protease;26 serine protease;endonuclease, polyU-specific;P11;placental protein 11;poly(U)-specific endoribonuclease;PP11;protein endoU;PRSS26;uridylate-specific endoribonuclease.
NCBI Gene Id8909
Protein NameP21128
Description of TargetPoly(U)-specific endoribonuclease
Uniprot IDhttps://www.uniprot.org/uniprot/P21128
Protein Accession #NP_001165910.1
Nucleotide Accession #NM_001172439.1
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