Catalog No: OKEH08606
Size:96T
Price: $725.00
SKU
OKEH08606
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology ENDOU ELISA Kit (OKEH08606) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ENDOU antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ENDOU and incubated. The ENDOU found in the sample or standards competes with the biotinylated ENDOU for limited binding sites on the immobilized anti-ENDOU antibody. Excess unbound biotinylated ENDOU and sample or standard ENDOU is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ENDOU captured in the well and inversely proportional to the amount of ENDOU which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.7% (n = 20) Mean Inter-assay CV%: <=10.4% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini. | ||||||||||||||||||||||
:: | Subcellular Location: Secreted | ||||||||||||||||||||||
:: | Unigene: Hs.997 SMR: P21128 String: 9606.ENSP00000397679 | ||||||||||||||||||||||
:: | KEGG: hsa:8909 MIM: 606720 Pfam: PF01033 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.25ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Poly(U)-specific endoribonuclease | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | ENDOU |
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Gene Full Name | endonuclease, poly(U) specific |
Alias Symbols | 22 serine protease;26 serine protease;endonuclease, polyU-specific;P11;placental protein 11;poly(U)-specific endoribonuclease;PP11;protein endoU;PRSS26;uridylate-specific endoribonuclease. |
NCBI Gene Id | 8909 |
Protein Name | P21128 |
Description of Target | Poly(U)-specific endoribonuclease |
Uniprot ID | https://www.uniprot.org/uniprot/P21128 |
Protein Accession # | NP_001165910.1 |
Nucleotide Accession # | NM_001172439.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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