EndoPDI Antibody (OASE00149)

Catalog No: OASE00149

Size:100ug

Immunoprecipitation analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57. Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:200. Courtesy of: Merrie Mosedale.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 2E7/7
• Isotype: IgG2a
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, ELISA
• Additional Information: Background Info: Detects a protein with a mass of ~48kDa corresponding to EndoPDI
  Scientific Background: Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of EndoPDI in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~48kDa.
• Dilution: WB (1:200); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Sullivan D.C., et al. (2003) J Biol Chem. 278 (47) 47079-47088.
• Description: Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).

Target Info

• Gene Symbol: PDI
• Gene Full Name: thioredoxin domain containing 5 (endoplasmic reticulum)
• Alias Symbols: Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras
• NCBI Gene Id: 81567
• Uniprot ID: Q8NBS9
• Protein Accession #: NP_001139021