EN2 Antibody - C-terminal region (OAAB04495)

Catalog No: OAAB04495

Size:400ul

Western blot analysis of EN2 antibody (C-term-2) in NCI-H460 cell lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain with EN2 Antibody (C-term-2), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EN2 Antibody (C-term-2) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC, IHC-P
• Additional Information: Cellular Location: Nucleus.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: EN2 Antibody (C-term-2) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: EN2 Antibody (C-term-2) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: EN2
• Alias Symbols: Homeobox protein engrailed-2, Hu-En-2 , EN2
• NCBI Gene Id: 2020
• Protein Name: Homeobox protein engrailed-2
• Description of Target: EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.
• Uniprot ID: P19622
• Protein Accession #: NP_001418.2
• Molecular Weight: 34211 Da