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YWHAG ELISA Kit (Chicken) (OKCA01039)

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96 Wells
$665.00
In Stock
Gene Symbol:
YWHAG
Official Gene Full Name:
14-3-3 protein gamma
NCBI Gene Id:
427820
Alias Symbols:
14-3-3GAMMA, 14-3-3 gamma
Description of Target:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Protein Name:
14-3-3 protein gamma
Swissprot Id:
Q5F3W6
Protein Accession #:
NP_001026648.1
Nucleotide Accession #:
NM_001031477.1
Species Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
0.039 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01039 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01039 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology YWHAG ELISA Kit (Chicken) (OKCA01039) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for YWHAG has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for YWHAG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample YWHAG captured in the well.
Kit Component:
ComponentAmount
YWHAG Microplate96 Wells (12 x 8 Well strips)
YWHAG Lyophilized Standard2 x 10 ng
100X Biotinylated YWHAG Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat9285-100
1:28981-99
1:49589-100
1:89085-98
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9582-105
EDTA Plasma (n=4)9385-100
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express YWHAG.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express YWHAG.
Datasheets/Manuals:
Printable datasheet/reference manual for OKCA01039
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
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