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WT1 Antibody - middle region (Phospho-E361) (OAAB08206)

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400ul
$320.00
In Stock
Gene Symbol:
WT1
NCBI Gene Id:
7490
Protein Name:
Wilms tumor protein
Swissprot Id:
P19544
Protein Accession #:
NP_000369.3
Alias Symbols:
WT1, Wilms tumor protein, WT33
Replacement Item:
This antibody may replace item sc-15421 from Santa Cruz Biotechnology.
Description of Target:
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight:
49188 Da
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB, IHC, IF, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express WT1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express WT1.
Species Reactivity:
Human, Mouse, Pig, Rat
Predicted Homology Based on Immunogen Sequence:
Human; Mouse; Pig; Rat
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets / Downloads:
OAAB08206
Specificity:
This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-383 amino acids from the Central region of human WT1.
Isotype:
Ig
Application Info:
WB 1:100 - 500
IHC 1:50 - 100
IF 1:10 - 50
FC 1:10 - 50
Additional Information:
Precautions: WT1 Antibody (Center E361) is for research use only and not for use in diagnostic or therapeutic procedures.
:::
Function: Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA
:::
Cellular Location: Nucleus. Nucleus, nucleolus. Cytoplasm (By similarity). Note=Shuttles between nucleus and cytoplasm (By similarity) Isoform 4: Nucleus, nucleoplasm.
Tissue Location: Expressed in the kidney and a subset of hematopoietic cells
Protocol:
Tips Information:

Product Protocol: WT1 antibody tested with human MCF-7 by western blot (OAAB08206)

Please find the protocol used to generate the western blot data for item (OAAB08206) by going here

Product datasheet link: WT1 antibody (OAAB08206)

WT1 Antibody (Center E361) (Cat. #OAAB08206) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the WT1 antibody detected the WT1 protein (arrow).

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's WT1 antibody (OAAB08206) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: WT1 antibody tested with human Kidney in immunohistochemistry (IHC) (OAAB08206)

Please go here to see the protocol used for Aviva's WT1 antibody (OAAB08206)

Product datasheet can be found here: WT1 antibody (OAAB08206)

IHC information:
WT1 Antibody (Center E361) (Cat. #OAAB08206) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's WT1 antibody (OAAB08206) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

 
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