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TPH2 ELISA Kit (Human) : 96 Wells (OKEH01578)

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96 Wells
$420.00
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Gene Symbol:
TPH2
Official Gene Full Name:
tryptophan hydroxylase 2
NCBI Gene Id:
121278
Alias Symbols:
ADHD7, FLJ37295, MGC138871, MGC138872, Neuronal tryptophan hydroxylase, NTPH, Tryptophan 5-hydroxylase 2, Tryptophan 5-monooxygenase 2
Description of Target:
This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression
Protein Name:
Tryptophan 5-hydroxylase 2
Swissprot Id:
Q8IWU9
Protein Accession #:
NP_775489.2
Nucleotide Accession #:
NM_173353.3
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 0.18 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.0% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology TPH2 ELISA Kit (Human) (OKEH01578) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TPH2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TPH2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TPH2 captured in well.
Kit Component:
ComponentAmount
TPH2 Microplate96 Wells (12 x 8 Well strips)
TPH2 Lyophilized Standard2
100X Biotinylated TPH2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TPH2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TPH2.
Datasheets / Downloads:
Printable datasheet for OKEH01578
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human Tryptophan 5-hydroxylase 2. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
56 kDa
Protein Size (# AA):
490
Protocol:
Tips Information:
 
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