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TNFRSF14/HVEM ELISA Kit (Human) (OKBB00550)

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96 Wells
$385.00
In Stock
Application:
ELISA
Gene Symbol:
TNFRSF14
Official Gene Full Name:
Protein Tnfrsf14
NCBI Gene Id:
8764
Alias Symbols:
ATAR, Herpesvirus entry mediator A, Herpes virus entry mediator A, HveA, HVEA, HVEM, LIGHTR, TR2, Tumor necrosis factor receptor-like 2, Tumor necrosis factor receptor superfamily member 14, UNQ329/PRO509
Description of Target:
Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
Protein Name:
Tumor necrosis factor receptor superfamily member 14
Swissprot Id:
Q92956
Protein Accession #:
NP_003811.2
Nucleotide Accession #:
NM_003820.3
Species Reactivity:
Human
Sample Type:
Cell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity:
<= 1 pg/mL
Kit Range:
15.6 pg/mL-1,000 pg/mL
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)6728462687324682
SD2.689.9432.64.6115.5541.6
CV%43.55.25.34.86.1
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology TNFRSF14/HVEM ELISA Kit (Human) (OKBB00550) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A mouse monoclonal antibody specific for TNFRSF14 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO; L39-V202) and test samples are added to the wells and incubated. After washing, abiotinylated polyclonal goat detector antibody specific for TNFRSF14 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human TNFRSF14 captured in well.
Kit Component:
ComponentAmount
96-Well plate Pre-coated with Anti-Human TNFRSF14 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human TNFRSF14 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human TNFRSF14 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TNFRSF14.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TNFRSF14.
Datasheets / Downloads:
Printable datasheet for OKBB00550
Reconstitution and Storage:
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles. Shipped with wet ice.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protocol:
Tips Information:
 
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