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TNFRSF11B ELISA Kit (Rat) (OKEH02974)

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96 Wells
$365.00
In Stock
Gene Symbol:
TNFRSF11B
Official Gene Full Name:
tumor necrosis factor receptor superfamily, member 11b
NCBI Gene Id:
25341
Alias Symbols:
MGC93568, Opg, Osteoprotegerin, Tumor necrosis factor receptor superfamily member 11B
Description of Target:
Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Protein Name:
Tumor necrosis factor receptor superfamily member 11B
Swissprot Id:
O08727
Protein Accession #:
NP_037002.1
Nucleotide Accession #:
NM_012870.2
Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 3.90 pg/mL
Kit Range:
15.6 - 1,000 pg/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology TNFRSF11B ELISA Kit (Rat) (OKEH02974) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfrsf11b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Tnfrsf11b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf11b captured in well.
Kit Component:
ComponentAmount
Anti-Tnfrsf11b Microplate96 Wells (12 x 8 Well strips)
Tnfrsf11b Lyophilized Standard2
100X Biotinylated Tnfrsf11b Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Tnfrsf11b.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Tnfrsf11b.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat Tumor necrosis factor receptor superfamily member 11B. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
46 kDa
Protein Size (# AA):
401
Protocol:
Tips Information:
 
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