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TNFRSF10B ELISA Kit (Human) : 96 Wells (OKEH01884)

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96 Wells
$480.00
In Stock
Gene Symbol:
TNFRSF10B
Official Gene Full Name:
tumor necrosis factor receptor superfamily member 10b
NCBI Gene Id:
8795
Alias Symbols:
CD262, Death receptor 5, DR5, KILLER, KILLER/DR5, TNF-related apoptosis-inducing ligand receptor 2, TRAILR2, TRAIL-R2, TRAIL receptor 2, TRICK2, TRICK2A, TRICK2B, TRICKB, Tumor necrosis factor receptor superfamily member 10B, UNQ160/PRO186, ZTNFR9
Description of Target:
The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene.
Protein Name:
Tumor necrosis factor receptor superfamily member 10B
Swissprot Id:
O14763
Protein Accession #:
NP_003833.4
Nucleotide Accession #:
NM_003842.4
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.12 ng/mL
Kit Range:
0.31 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology TNFRSF10B ELISA Kit (Human) (OKEH01884) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF10B has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF10B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TNFRSF10B captured in well.
Kit Component:
ComponentAmount
TNFRSF10B Microplate96 Wells (12 x 8 Well strips)
TNFRSF10B Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ABO Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TNFRSF10B.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TNFRSF10B.
Datasheets / Downloads:
Printable datasheet for OKEH01884
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human TNFRSF10B. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
48 kDa
Protein Size (# AA):
440
Protocol:
Tips Information:
 
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