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TCP1-alpha Antibody (OASE00325)

100ug
$325.00
In Stock

Conjugation Options

OASE00325-FITC Conjugated

OASE00325-HRP Conjugated

OASE00325-Biotin Conjugated

Gene Symbol:
Tcp1
NCBI Gene Id:
21454
Official Gene Full Name:
t-complex protein 1
Swissprot Id:
P11983
Protein Accession #:
NP_038714.2
Replacement Item:
This antibody may replace item sc-13869 from Santa Cruz Biotechnology.
Host:
Rat
Clonality:
Monoclonal
Purification:
Protein G Purified
Application:
WB, IP, FC, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Tcp1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Tcp1.
Immunogen:
Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Species Reactivity:
Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Monkey
Reconstitution and Storage:
Store at -20C. Shipping in Blue Ice or 4C.
Concentration:
1mg/mL
Datasheets/Manuals:
Printable datasheet for OASE00325
Dilution:
1:1000 (WB)
Clone:
91a
Isotype:
IgG2a
Additional Information:
Background Info: Detects a 60kDa protein corresponding to the molecular weight of TCP1 alpha; also detects a molecular mass band of ~92kDa. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
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Scientific Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60,Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
:::
Certificate of Analysis: 1ug/mL was sufficient for detection of TCP1 alpha in 20 ug of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Protocol:
Tips Information:

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