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TAS2R38 ELISA Kit (Human) (OKEH02207)

96 Wells
$430.00
In Stock
Application:
ELISA
Gene Symbol:
TAS2R38
Official Gene Full Name:
taste 2 receptor member 38
NCBI Gene Id:
5726
Alias Symbols:
PTC, PTC bitter taste receptor, T2R38, T2R61, Taste receptor type 2 member 38, Taste receptor type 2 member 61
Description of Target:
This gene encodes a seven-transmembrane G protein-coupled receptor that controls the ability to taste glucosinolates, a family of bitter-tasting compounds found in plants of the Brassica sp. Synthetic compounds phenylthiocarbamide (PTC) and 6-n-propylthiouracil (PROP) have been identified as ligands for this receptor and have been used to test the genetic diversity of this gene. Although several allelic forms of this gene have been identified worldwide, there are two predominant common forms (taster and non-taster) found outside of Africa. These alleles differ at three nucleotide positions resulting in amino acid changes in the protein (A49P, A262V, and V296I) with the amino acid combination PAV identifying the taster variant (and AVI identifying the non-taster variant).
Protein Name:
Taste receptor type 2 member 38
Swissprot Id:
P59533
Protein Accession #:
NP_789787.4
Nucleotide Accession #:
NM_176817.4
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.11 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology TAS2R38 ELISA Kit (Human) (OKEH02207) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human TAS2R38 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TAS2R38 and incubated. The TAS2R38 found in the sample or standards competes with the biotinylated TAS2R38 for limited binding sites on the immobilized anti-Human TAS2R38 antibody. Excess unbound biotinylated TAS2R38 and sample or standard TAS2R38 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TAS2R38 captured in the well and inversely proportional to the amount of TAS2R38 which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-TAS2R38 Microplate96 Wells (12 x 8 Well strips)
TAS2R38 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X TAS2R38 Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TAS2R38.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TAS2R38.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural Human T2R38. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative competitive Immunoassay
Molecular Weight:
38 kDa
Protein Size (# AA):
333
Protocol:
Tips Information:

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