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Synuclein Alpha Antibody ELISA Kit (Human) (OKCD01295)

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96 Wells
$680.00
In Stock
Alias Symbols:
SNCA, alpha-synuclein, Alpha-synuclein, MGC110988, NACP, Non-A4 component of amyloid precursor, Non-A beta component of AD amyloid, PARK1, PARK4, PD1
Description of Target:
Synuclein Alpha antibody
Protein Name:
Synuclein Alpha
Swissprot Id:
P37840
Nucleotide Accession #:
NM_000345.3
Species Reactivity:
Human
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
1.17 ng/mL
Kit Range:
3.12-200 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Synuclein Alpha antibody were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Synuclein Alpha antibody were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology Synuclein Alpha antibody ELISA Kit (Human) (OKCD01295) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. A Synuclein Alpha antibody has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. An HRP conjugated detector antibody specific for Human antibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SNCa Antibody captured in well.
Kit Component:
ComponentAmount
Synuclein Alpha antibody Microplate96 Wells (12 x 8 Well strips)
Synuclein Alpha antibody Lyophilized Standard2 x XXX
100X Detector Antibody1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)90-97%89-96%96-103%98-105%
EDTA Plasma (n=5)79-103%80-90%88-101%83-99%
Heparin Plasma (n=5)82-89%82-91%86-95%80-89%
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)85-10393
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)90-9794
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets / Downloads:
Printable datasheet for OKCD01295
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Method: Quantitative Reverse Capture Sandwich ELISA
Protocol:
Tips Information:
 
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