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SPRN ELISA Kit (Mouse) (OKCA01773)

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96 Wells
$600.00
In Stock
Gene Symbol:
SPRN
Official Gene Full Name:
shadow of prion protein
NCBI Gene Id:
212518
Alias Symbols:
FLJ41197, SHADOO, SHO, bA108K14.1, shadow of prion protein
Description of Target:
Prion-like protein that has PrP(C)-like neuroprotective activity. May act as a modulator for the biological actions of normal and abnormal PrP.
Protein Name:
Shadow of prion protein
Swissprot Id:
Q8BWU1
Protein Accession #:
NP_898970.1
Nucleotide Accession #:
NM_183147.2
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
0.039 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology Sprn ELISA Kit (Mouse) (OKCA01773) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sprn has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sprn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sprn captured in the well.
Kit Component:
ComponentAmount
Sprn Microplate96 Wells (12 x 8 Well strips)
Sprn Lyophilized Standard2 x 10 ng
100X Biotinylated Sprn Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9590-100
EDTA Plasma (n=4)9288-96
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Sprn.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Sprn.
Datasheets / Downloads:
Printable datasheet for OKCA01773
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
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