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Spn ELISA Kit (Mouse) (OKEH04706)

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96 Wells
$425.00
In Stock
Gene Symbol:
SPN
Official Gene Full Name:
Sialophorin
NCBI Gene Id:
20737
Alias Symbols:
3E8 antigen, A630014B01Rik, B-cell differentiation antigen LP-3, Cd43, Galgp, Leukocyte sialoglycoprotein, leukosialin, Leukosialin, Ly48, Ly-48, Lymphocyte antigen 48, Sialophorin
Description of Target:
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
Protein Name:
Leukosialin
Swissprot Id:
P15702
Protein Accession #:
NP_001032899.1
Nucleotide Accession #:
NM_001037810.2
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity:
< 0.15 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology Spn ELISA Kit (Mouse) (OKEH04706) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Spn has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Spn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Spn captured in well.
Kit Component:
ComponentAmount
Spn Microplate96 Wells (12 x 8 Well Strips)
Spn Standard2
Sample Diluent1 x 20 mL
Spn Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SPN.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SPN.
Datasheets / Downloads:
Printable datasheet for OKEH04706
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural mouse Leukosialin. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
40 kDa
Protein Size (# AA):
395
Protocol:
Tips Information:
 
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