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SNCG ELISA Kit (Human) (OKEH04808)

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96 Wells
$425.00
In Stock
Gene Symbol:
SNCG
Official Gene Full Name:
Synuclein gamma
NCBI Gene Id:
6623
Alias Symbols:
SR, BCSG1
Description of Target:
This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development.
Protein Name:
Gamma-synuclein
Swissprot Id:
O76070
Protein Accession #:
NP_001317049.1
Nucleotide Accession #:
NM_001330120.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity:
< 0.39 ng/mL
Kit Range:
0.78 - 50 ng/mL
Kit Reproducibility:
Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology SNCG ELISA Kit (Human) (OKEH04808) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNCG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNCG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNCG captured in well.
Kit Component:
ComponentAmount
SNCG Microplate96 Wells (12 x 8 Well Strips)
SNCG Standard2
Sample Diluent1 x 20 mL
SNCG Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SNCG.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SNCG.
Datasheets / Downloads:
Printable datasheet for OKEH04808
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human Gamma-synuclein. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
13 kDa
Protein Size (# AA):
127
Protocol:
Tips Information:
 
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