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SNCA ELISA Kit (Pig) (OKEH03912)

96 Wells
$495.00
In Stock
Gene Symbol:
SNCA
Official Gene Full Name:
synuclein, alpha (non A4 component of amyloid precursor)
NCBI Gene Id:
641350
Alias Symbols:
Alpha-synuclein
Description of Target:
May be involved in the regulation of dopamine release and transport.
Protein Name:
Alpha-synuclein
Swissprot Id:
Q3I5G7
Protein Accession #:
NP_001032222.1
Nucleotide Accession #:
NM_001037145.1
Species Reactivity:
Pig
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 7.8 pg/mL
Kit Range:
15.6 - 1,000 pg/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology SNCA ELISA Kit (Pig) (OKEH03912) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNCA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNCA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNCA captured in well.
Kit Component:
ComponentAmount
SNCA Microplate96 Wells (12 x 8 Well strips)
SNCA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SNCA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SNCA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SNCA.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural pig Alpha-synuclein. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
140 kDa
Protein Size (# AA):
140
Protocol:
Tips Information:

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