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SNCA ELISA Kit (Mouse) (OKCD02898)

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96 Wells
$535.00
In Stock
Gene Symbol:
SNCA
Official Gene Full Name:
synuclein, alpha
NCBI Gene Id:
20617
Alias Symbols:
alphaSYN, alpha-synuclein, Alpha-synuclein, NACP, Non-A4 component of amyloid precursor, Non-A beta component of AD amyloid, Syn
Description of Target:
May be involved in the regulation of dopamine release and transport.
Protein Name:
Alpha-synuclein
Swissprot Id:
O55042
Protein Accession #:
NP_001035916.1
Nucleotide Accession #:
NM_001042451.2
Species Reactivity:
Mouse
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Sensitivity:
28 pg/mL
Kit Range:
78-5,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Snca was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Snca was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Duration:
3 Hours
Kit Principle:
Aviva Systems Biology Snca ELISA Kit (Mouse) (OKCD02898) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Snca has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Snca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Snca captured in well.
Kit Component:
ComponentAmount
Anti-Snca Microplate96 Wells (12 x 8 Well strips)
Snca Lyophilized Standard2
100X Biotinylated Snca Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)78-103%78-101%97-105%91-99%
EDTA Plasma (n=5)97-105%86-104%90-97%89-103%
Heparin Plasma (n=5)86-102%91-104%95-102%78-97%
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)95-104101
EDTA Plasma (n=5)87-9690
Heparin Plasma(n=5)86-10598
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SNCA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SNCA.
Datasheets / Downloads:
Printable datasheet for OKCD02898
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Synuclein Alpha (SNCa). No significant cross-reactivity or interference between Synuclein Alpha (SNCa) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Protein Size (# AA):
140
Protocol:
Tips Information:
 
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