Now Offering Over 102,157 Antibodies & 44,722 Antigens!

SNAP25 ELISA Kit (Mouse) (OKEH03377)

96 Wells
$435.00
In Stock
Gene Symbol:
SNAP25
Official Gene Full Name:
synaptosomal-associated protein 25
NCBI Gene Id:
20614
Alias Symbols:
Bdr, GENA 70, Snap, SNAP-25, sp, SUP, Super protein, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25
Description of Target:
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF (PubMed:16672379). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Protein Name:
Synaptosomal-associated protein 25(SNAP-25)
Swissprot Id:
P60879
Protein Accession #:
NP_001277985.1
Nucleotide Accession #:
NM_001291056.1
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.156 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 6.2% (n = 20)
Mean Inter-assay CV%: <= 8.7% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology SNAP25 ELISA Kit (Mouse) (OKEH03377) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Snap25 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Snap25 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Snap25 captured in well.
Kit Component:
ComponentAmount
Anti-Snap25 Microplate96 Wells (12 x 8 Well strips)
Snap25 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Snap25 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Snap25.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Snap25.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural mouse SNAP-25. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
23 kDa
Protein Size (# AA):
206
Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...