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SCARB1 ELISA Kit (Human) (OKEH04223)

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96 Wells
$596.00
In Stock
Gene Symbol:
SCARB1
Official Gene Full Name:
scavenger receptor class B member 1
NCBI Gene Id:
949
Alias Symbols:
CD36 and LIMPII analogous 1, CD36 antigen-like 1, CD36L1, CLA1, CLA-1, Collagen type I receptor, thrombospondin receptor-like 1, HDLQTL6, MGC138242, Scavenger receptor class B member 1, SRB1, SR-BI
Description of Target:
The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene.
Protein Name:
scavenger receptor class B member 1
Swissprot Id:
Q8WTV0
Protein Accession #:
NP_001076428.1
Nucleotide Accession #:
NM_001082959.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity:
< 0.15 ng/mL
Kit Range:
0.31 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 4.7% (n = 20)
Mean Inter-assay CV%: <= 8.6% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology SCARB1 ELISA Kit (Human) (OKEH04223) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCARB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCARB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SCARB1 captured in the well.
Kit Component:
ComponentAmount
SCARB1 Microplate96 Wells (12 x 8 Well Strips)
SCARB1 Standard2 x 2 ng
Sample Diluent1 x 20 mL
SCARB1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SCARB1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SCARB1.
Datasheets / Downloads:
Printable datasheet for OKEH04223
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human SRB1. No significant cross-reactivity or interference was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
61 kDa
Protein Size (# AA):
552
Protocol:
Tips Information:
 
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