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ROMO1 ELISA Kit (Human) (OKEH01371)

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96 Wells
$420.00
In Stock
Application:
ELISA
Gene Symbol:
ROMO1
Official Gene Full Name:
reactive oxygen species modulator 1
NCBI Gene Id:
140823
Alias Symbols:
bA353C18.2, C20orf52, MGC111180, MTGMP, Protein MGR2 homolog, Reactive oxygen species modulator 1, ROS modulator 1
Description of Target:
The protein encoded by this gene is a mitochondrial membrane protein that is responsible for increasing the level of reactive oxygen species (ROS) in cells. The protein also has antimicrobial activity against a variety of bacteria by inducing bacterial membrane breakage.
Protein Name:
Reactive oxygen species modulator 1
Swissprot Id:
P60602
Protein Accession #:
NP_542786.1
Nucleotide Accession #:
NM_080748.2
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 0.078 ng/mL
Kit Range:
0.15 - 10 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology ROMO1 ELISA Kit (Human) (OKEH01371) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ROMO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ROMO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ROMO1 captured in well.
Kit Component:
ComponentAmount
ROMO1 Microplate96 Wells (12 x 8 Well strips)
ROMO1 Lyophilized Standard2
100X Biotinylated ROMO1 Detector Antibody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ROMO1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ROMO1.
Datasheets / Downloads:
Printable datasheet for OKEH01371
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human ROS modulator 1. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
8 kDa
Protein Size (# AA):
79
Protocol:
Tips Information:
 
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