Now Offering Over 102,157 Antibodies & 44,722 Antigens!

ROCK2 ELISA Kit (Human) (OKCD07732)

* Required Fields

$0.00
In Stock
Application:
ELISA
Gene Symbol:
ROCK2
Official Gene Full Name:
Rho associated coiled-coil containing protein kinase 2
NCBI Gene Id:
9475
Alias Symbols:
ROCK-II
Description of Target:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Protein Name:
Rho-associated protein kinase 2
Swissprot Id:
O75116
Protein Accession #:
NM_001321643.1
Nucleotide Accession #:
NP_001308572.1
Species Reactivity:
Human
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
< 0.061ng/mL
Kit Range:
0.156-10ng/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level ROCK2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level ROCK2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology ROCK2 ELISA Kit (Human) (OKCD07732) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ROCK2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ROCK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ROCK2 captured in the well.
Kit Component:
ComponentAmount
ROCK2 Microplate96 Wells (12 x 8 Well strips)
ROCK2 Lyophilized Standard2
100X ROCK2 HRP-Detector Anitbody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by RNA-seq analysis to express ROCK2.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express ROCK2.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...