Now Offering Over 102,157 Antibodies & 44,722 Antigens!

ROCK1 ELISA Kit (Human) (OKEH06554)

Print Page
96 Wells
$480.00
In Stock
Gene Symbol:
ROCK1
NCBI Gene Id:
6093
Alias Symbols:
MGC131603, MGC43611, p160ROCK, P160ROCK, p160 ROCK-1, PRO0435, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated protein kinase 1
Description of Target:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.1
Protein Name:
Rho-associated protein kinase 1
Swissprot Id:
Q13464
Protein Accession #:
NP_001265491
Nucleotide Accession #:
NM_001278562
Species Reactivity:
Human
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
0.625 ng/mL
Kit Range:
0.625-40 ng/mL
Kit Reproducibility:
Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 7.1% (n = 20)
Mean Inter-Assay Precision - <= 8.4% (n = 20)
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology ROCK1 ELISA Kit (Human) (OKEH06554) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ROCK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ROCK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ROCK1 captured in the well.
Kit Component:
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Recovery:
Mean recovery when spiking into Serum and Plasma = 91%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ROCK1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ROCK1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Protocol:
Tips Information:
 
X
Ask a Question

Tell us what you think about this item!

Write A Review
    Please, wait...