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TRAK1 antibody - middle region (ARP54521_P050)

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100 ul
$289.00
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Conjugation Options

ARP54521_P050-FITC Conjugated

ARP54521_P050-HRP Conjugated

ARP54521_P050-Biotin Conjugated

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Gene Symbol:
TRAK1
NCBI Gene Id:
22906
Official Gene Full Name:
Trafficking protein, kinesin binding 1
Protein Name:
Trafficking kinesin-binding protein 1
Swissprot Id:
Q9UPV9
Protein Accession #:
NP_001036111
Nucleotide Accession #:
NM_001042646
Alias Symbols:
OIP106, MILT1
Description of Target:
The specific function of the protein remains unknown.
Protein Size (# AA):
953
Molecular Weight:
106kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TRAK1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TRAK1.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human TRAK1
Species Reactivity:
Cow, Dog, Horse, Human, Pig, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 93%; Dog: 93%; Horse: 93%; Human: 100%; Pig: 93%; Rabbit: 83%; Rat: 79%
Complete computational species homology data:
Anti-TRAK1 (ARP54521_P050)
Peptide Sequence:
Synthetic peptide located within the following region: ILETEAADLGNDERSKKPGTPGTPGSHDLETALRRLSLRRENYLSERRFF
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
SKIL; CDK6; HGS; TRAF3IP1; CDC5L; TRAK1; POLR2A; OGT; TRAK2; KIF5B; KIF5C;
Blocking Peptide:
For anti-TRAK1 (ARP54521_P050) antibody is Catalog # AAP54521 (Previous Catalog # AAPP31305)
Datasheets/Manuals:
Printable datasheet for anti-TRAK1 (ARP54521_P050) antibody
Sample Type Confirmation:

TRAK1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

There is BioGPS gene expression data showing that TRAK1 is expressed in HCT15, HT1080

TRAK1 is supported by BioGPS gene expression data to be expressed in H

Target Reference:
Fransson,S., (2006) Biochem. Biophys. Res. Commun. 344 (2), 500-510
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02/01/2017 16:24
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Product Review: TRAK1 antibody-middle region (ARP54521_P050) in myc-hTRAK1 transfected HEK293 lysate using Western blot
Product Page for TRAK1 antibody-middle region (ARP54521_P050)

Researcher: Gulcin Pekkurnaz, F.M. Kirby Neurobiology Center
Application: Western blotting
Species + Tissue/Cell type: Lane1: 10 ug myc-hTRAK1 transfected HEK293 lysate
Primary antibody dilution: 1:500
Secondary antibody: Anti-rabbit HRP
Secondary antibody dilution: 1:2000



Questionnaire:

How do Aviva's reagents play a role in your experimental goals?

I tested the antibody recently and I am planning to use it in the future experiments.

How would you rate this antibody on a scale from 1-5 (5=best) and why?

4

Would you use this antibody in future experiments?

YES

Have you used another antibody which has worked in your application?

YES

Do you believe the information about the reagent on Aviva's website is correct?

YES

Validation: Western Blot

Sample Description: Myc-tagged hMilton1 overexpressed HEK293T lysate

Species, Tissue/Cell Type: Human cell line

How many different experimental trials were conducted using the antibody sample?

1

What type of experimental sample are you using and how did you preparing it?

2x106 HEK cells were solubilized in 600ul lysis buffer, resuspended in Laemmli  buffer. 2.5% of the cell lysate used for WB.

Primary used and dilution: 1:500 in 5%milk PBS

Secondary used and dilution: 1:5000 in 5%milk PBS

Experimental Procedure/Protocols:

8% gel, standart WB protocol.

What controls were used in your experiment? Please include your positive control:

Overexpressed protein was also myc-tagged. Anti-myc Ab used as a positive control

How did you store the antibody after re-suspension?

-20C
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