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SOD2 antibody - N-terminal region (ARP58529_P050)

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100 ul

Regular Price: $289.00

Special Price: $215.00

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Conjugation Options

ARP58529_P050-FITC Conjugated

ARP58529_P050-HRP Conjugated

ARP58529_P050-Biotin Conjugated

Gene Symbol:
SOD2
NCBI Gene Id:
6648
Official Gene Full Name:
Superoxide dismutase 2, mitochondrial
Protein Name:
Superoxide dismutase [Mn], mitochondrial
Swissprot Id:
P04179
Protein Accession #:
NP_000627
Nucleotide Accession #:
NM_000636
Alias Symbols:
IPO-B, MNSOD, Mn-SOD, IPOB, MVCD6
Replacement Item:
This antibody may replace item sc-113078 from Santa Cruz Biotechnology.
Description of Target:
SOD2 is a member of the iron/manganese superoxide dismutase family. SOD2 is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene encoding SOD2 have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Protein Size (# AA):
222
Molecular Weight:
24kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SOD2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SOD2.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human SOD2
Species Reactivity:
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 86%
Complete computational species homology data:
Anti-SOD2 (ARP58529_P050)
Peptide Sequence:
Synthetic peptide located within the following region: MLSRAVCGTSRQLAPVLGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLH
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
CYP4F12; UBC; SOD2; SOD1; APP; NABP2; TBL2; ZC3H11A; VAPA; STX7; UBL4A; ALDH5A1; USP36; H2AFX; SUMO1; NOL12; HDHD2; GET4; RPS3A; RAB4A; AURKA;
Blocking Peptide:
For anti-SOD2 (ARP58529_P050) antibody is Catalog # AAP58529 (Previous Catalog # AAPP34826)
Datasheets / Downloads:
Printable datasheet for anti-SOD2 (ARP58529_P050) antibody
Target Reference:
Martin,R.C., (2008) DNA Cell Biol. 27 (6), 321-323
Additional Product Images:

Product Protocols: SOD2 antibody tested with Human Placenta Tissue (ARP58529_P050)

Aviva Systems Biology is the original manufacturer of this SOD2 antibody (ARP58529_P050)

Click here to view the SOD2 antibody Western Blot Protocol

Product Datasheet Link: SOD2 antibody (ARP58529_P050)

WB Suggested Anti-SOD2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Placenta

Western Blot image:


Description of Target: SOD2 is a member of the iron/manganese superoxide dismutase family. SOD2 is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene encoding SOD2 have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s SOD2 antibody (ARP58529_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Review: SOD2 antibody – N-terminal region (ARP58529_P050) with HEK293T cells using Western Blot

Product Page Link: SOD2 antibody - N-terminal region (ARP58529_P050)

Data provided by: Dr. Giovambattista Panipani

Tissue homogenates and cell lysates were prepared by standard procedures, suitable for analysis of other proteins or even of the same proteins with different specific reagents.

Primary dilution 1:500; Secondary dilution 1:5-10000

 
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2 Item(s)

02/01/2017 16:24
  • Overall Experience:
  • Quality:
Product Review: SOD2 antibody - N-terminal region (ARP58529_P050) in Human HK2 cell (kidney proximal tubular cell line) using Western Blot
Product Page for SOD2 antibody - N-terminal region (ARP58529_P050)

Researcher: The University of Queensland, School of Medicine, Centre for Kidney Disease Research
Application: Western blotting
Species + Tissue/Cell type: Human HK2 cell (kidney proximal tubular cell line)
How many ug's of tissue/cell lysate run on the gel:
1. 40 ug HK2 cell (kidney proximal tubular cell line)
2. 40 ug H2O2 treated human HK2 Cell lysate
3. 40 ug H2O2 treated human HK2 Cell lysate
4. 40 ug H2O2 treated human HK2 Cell lysate
5. 40 ug H2O2 treated human HK2 Cell lysate
6. 40 ug H2O2 treated human HK2 Cell lysate
Primary antibody dilution: 1:1000
Secondary antibody: Goat anti-Rabbit HRP
Secondary antibody dilution: 1:2000



Questionnaire:
How do Aviva's reagents play a role in your experimental goals? Determine their expression levels in response to oxidative stress.
How would you rate this antibody on a scale from 1-5 (5=best) and why? 4.5 - very specific band found at 24 kDa.
Would you use this antibody in future experiments? Yes
Have you used another antibody which has worked in your application? Yes, but your antibody produced a cleaner result.
Do you believe the information about the reagent on Aviva's website is correct? Yes
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? Yes, SOD2 expresson is very important in oxidative stress research.
How did you store the antibody after re-suspension? Aliquoted for single use at -20 degree Celsius.
Sample Description, Species, Tissue/Cell Type: Human. HK2 cell (kidney proximal tubular cell line). 40 ug
How many different experimental trials were conducted using the antibody sample? 1
What type of experimental sample are you using and how did you preparing it? HK2 cells were grown in 10cm cell culture dishes in Dulbecco's modified Eagle's Medium: Ham's F-12 (DMEM/F12) containing antibiotics Penicillin (1000 U/ml) and Streptomycin (1000 ug/ml), and fetal bovine serum (FBS) and allowed 24h to adehere. Cells were exposed to hydrogen peroxide (H2O2) diluted in growth media at 0.2, 0.4, 0.6. 0.8, 1.0 mM for 2h.
Primary used and dilution: 1:1000; overnight (approx 18h) at 4 degree celsius.
Secondary used and dilution: Goat anti-rabbit IgG Horseradish peroxidate conjugate(G21234) (Molecular Probes-Invitrogen), 1:2000, 1h at RT.
What controls were used in your experiment? Please include your positive control: Untreated cells.
Experimental Procedure/Protocols: Cells were placed on ice and washed X3 with cold phosphate buffered saline (PBS), then scraped into radio-immunoprecipitation assay (RIPA) cell lysis buffer (0.15 M sodium chloride, 0.025 M sodium fluoride, 0.5 M ethylenediaminetetraacetic acid), 0.1% sodium dodecylsulphate (SDS), 1.0% lgepal in 50 mM Tris-Cl, pH 7.5) containing phosphatase and protease inhibitors (10 ug/mL aprotinin, 10 ug/mL leupeptin, 1mM sodium orthovanadate and 100 ug/mL phenylmethylsulfonyl chloride). After cell lysis by sonification, cell debris was removed by centrifugation (13,000 g, 20 min, 4 degree C). Protein concentration was determined using a Pierce bicinchoninic acid (BCA) protein assay and spectroscopy at 540 nm. Cell lysate was snap frozen in liquid nitrogen and stored at -80 degree c.40 ug of each protein were loaded into seperate lanes of a 10% sodium dodecylsulphate (SDS)-polyacrylamide gel and electrophoresed using Bio-Rad equipment, then electrophoretically transfered onto PVDF membrane.  Bio-Rad Precision Plus Protein Kaleidoscope (Cat#161-0375) was routinely used as a protein size marker. To inhibit non-specific binding, membranes were first soaked in a blocking solution of 5 % skim milk in Tris-buffered saline (TBS) with Tween-20 pH 7.4 (TBST) (5% blotto) for 1 hour. Membrane were blotted with the primary antibody provided. Primary antibodies were diluted in a 1% blotto solution and incubated for 18-24 h at 4 degree C. Membranes were washed X3 in TBST for 5 min. Goat anti-rabbit IgG-HRP-conjugated secondary antibody diluted at 1:2000 in 1% blotto was used for 1h at RT. Protein bands were visualised using enhanced chemiluminescence and X-ray flim. Developed flims were scanned using a Canon Canoscan 8400F at 300dpi.
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02/01/2017 16:24
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Product Review: SOD2 antibody - N-terminal region (ARP58529_P050) in rat dorsal medulla brain & cortax + hypothalamus using western blot
Product Page for SOD2 antibody - N-terminal region (ARP58529_P050)

Researcher: Manisha Nautiyal, Hypertension and Vascular Research Center, Wake Forest University School of Medicine
Application: Western blotting
Species + Tissue/Cell type: Rat dorsal medulla brain & cortax + hypothalamus extract
How many ug's of tissue/cell lysate run on the gel: 1. 40ug rat dorsal medulla brain extract 2. 20 ug rat cortax + hypothalamus mitochondria extract
Primary antibody dilution: 1:2500
Secondary antibody: Anti-Rabbit HRP
Secondary antibody dilution: 1:5000



Questionnaire:

How do Aviva's reagents play a role in your experimental goals?

To determine oxidative stress status in our animal models.

How would you rate this antibody on a scale from 1-5 (5=best) and why?

4- we see bands at expected MW about 24 kDa, however we do see some non-specific  bands at higher MWs.

Would you use this antibody in future experiments?

Yes.

Have you used another antibody which has worked in your application?

Yes.

If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not?

Yes.

Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel):

Rat brain: lane #1 is dorsal medulla tissue extract (40 ug) and lane # 2 is isolated mitochondria from rat brain cortex and hypothalamus pooled (20 ug).

How many different experimental trials were conducted using the antibody sample?

1

What type of experimental sample are you using and how did you preparing it?

"Lane # 1: homogenize brain dorsal medulla in 20mM potassium phosphate buffer, pH 7.0 with 1mM EGTA and Sigma protease cocktail inhibitor; and prepare samples in Laemmli buffer for WB

Lane # 2: isolate brain mitochondria (cortex and hypothalamus pooled) and prepare samples in Laemmli buffer for WB"

Primary used and dilution:

1: 2500 in 5% milk-TBST (0.05% Tween) for overnight at 4 degrees.

Secondary used and dilution:

1: 5000 anti-Rabbit HRP in 5% milk-TBST for 2 hrs at room temperature.

Experimental Procedure/Protocols:

"Gels: (cat#161-1155, BioRad) Ready Gel Tris-HCl Gel, 10% resolving gel, 4% stacking gel, 10-well, 50 ul,

Running buffer: BioRad cat #  161-0732   : 1X Tris/Glycine/SDS: run gels for 70 min at 120 V

Transfer buffer: BioRad cat #  161-0734   : 1X Tris/Glycine with 20% methanol: Transfer for 1 hr at 100V

Blocking buffer: 5% milk in TBS-Tween (0.05%) for 1 hr at room temperature

Primary antibody: overnight at 4 degrees

Wash 3 times with TBST for 10 min per wash

Secondary antibody: 2 hrs room temperature

Wash 3 times wiht TBST for 10 min per wash

Develop bands with chemiluminescent substrate (Pierce: SuperSignal West Pico Chemiluminescent Substrate)"

What controls were used in your experiment? Please include your positive control:

Tissue (positive), mitochondria (negative).

How did you store the antibody after re-suspension?

In water, at -80 degrees.
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