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RAP1A ELISA Kit (Rat) (OKEH03532)

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96 Wells
$455.00
In Stock
Gene Symbol:
RAP1A
Official Gene Full Name:
RAP1A, member of RAS oncogene family
NCBI Gene Id:
295347
Alias Symbols:
Krev1, MGC93785, MGC94873, rap 1A, RAP-1A, Ras-related protein Krev-1, Ras-related protein Rap-1A
Description of Target:
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
Protein Name:
Ras-related protein Rap-1A
Swissprot Id:
P62836
Protein Accession #:
NP_001005765.1
Nucleotide Accession #:
NM_001005765.1
Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.078 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology RAP1A ELISA Kit (Rat) (OKEH03532) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rap1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Rap1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rap1a captured in well.
Kit Component:
ComponentAmount
Rap1a Microplate96 Wells (12 x 8 Well strips)
Rap1a Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Rap1a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Rap1a.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Rap1a.
Datasheets / Downloads:
Printable datasheet for OKEH03532
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural rat Ras-related protein Rap-1A. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
21 kDa
Protein Size (# AA):
184
Protocol:
Tips Information:
 
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