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RAP1A ELISA Kit (Human) : 96 Wells (OKEH01696)

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96 Wells
$450.00
In Stock
Gene Symbol:
RAP1A
Official Gene Full Name:
RAP1A, member of RAS oncogene family
NCBI Gene Id:
5906
Alias Symbols:
C21KG, G-22K, GTP-binding protein smg p21A, KREV1, KREV-1, RAP1, Ras-related protein Krev-1, Ras-related protein Rap-1A, SMGP21
Description of Target:
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.
Protein Name:
Ras-related protein Rap-1A
Swissprot Id:
P62834
Protein Accession #:
NP_001010935.1
Nucleotide Accession #:
NM_001010935.2
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 0.056 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 3.6% (n = 20)
Mean Inter-assay CV%: < 7.0% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology RAP1A ELISA Kit (Human) (OKEH01696) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAP1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAP1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAP1A captured in well.
Kit Component:
ComponentAmount
RAP1A Microplate96 Wells (12 x 8 Well strips)
RAP1A Lyophilized Standard2
100X Biotinylated RAP1A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express RAP1A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express RAP1A.
Datasheets / Downloads:
Printable datasheet for OKEH01696
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human Ras-related protein Rap-1A. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
21 kDa
Protein Size (# AA):
184
Protocol:
Tips Information:
 
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