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PSAP ELISA Kit (Human) (OKCD02827)

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96 Wells
$545.00
In Stock
Gene Symbol:
PSAP
Official Gene Full Name:
prosaposin
NCBI Gene Id:
5660
Alias Symbols:
FLJ00245, GLBA, MGC110993, Proactivator polypeptide, SAP1
Description of Target:
Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12). Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.
Protein Name:
Prosaposin
Swissprot Id:
P07602
Protein Accession #:
NP_001035930.1
Nucleotide Accession #:
NM_001042465.2
Species Reactivity:
Human
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, seminal plasma, cell culture cell supernatant and other biological fluids
Sensitivity:
0.067 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level PSAP was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level PSAP was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Duration:
3 Hours
Kit Principle:
Aviva Systems Biology PSAP ELISA Kit (Human) (OKCD02827) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PSAP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for PSAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PSAP captured in well.
Kit Component:
ComponentAmount
Anti-PSAP Microplate96 Wells (12 x 8 Well strips)
PSAP Lyophilized Standard2
100X Biotinylated PSAP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)94-102%84-101%84-94%82-92%
EDTA Plasma (n=5)97-105%92-99%90-99%97-105%
Heparin Plasma (n=5)87-99%78-99%80-98%92-99%
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10288
EDTA Plasma (n=5)98-105102
Heparin Plasma(n=5)96-10499
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PSAP.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PSAP.
Datasheets / Downloads:
Printable datasheet for OKCD02827
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Prosaposin (PSAP). No significant cross-reactivity or interference between Prosaposin (PSAP) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Protein Size (# AA):
524
Protocol:
Tips Information:
 
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