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PRLR ELISA Kit (Mouse) (OKCA01745)

96 Wells
In Stock
Gene Symbol:
Official Gene Full Name:
prolactin receptor
NCBI Gene Id:
Alias Symbols:
hPRLrI, delta 4-SF1b truncated prolactin receptor|delta 4-delta 7/11 truncated prolactin receptor|hPRL receptor|prolactin receptor delta 7/11|secreted prolactin binding protein
Description of Target:
This is a receptor for the anterior pituitary hormone prolactin.
Protein Name:
Prolactin receptor
Swissprot Id:
Protein Accession #:
Nucleotide Accession #:
Species Reactivity:
Sample Type:
Serum, Plasma, Tissue Homogenates
2.35 pg/mL
Kit Range:
9.38 - 600 pg/mL
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology Prlr ELISA Kit (Mouse) (OKCA01745) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prlr has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prlr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Prlr captured in the well.
Kit Component:
Prlr Microplate96 Wells (12 x 8 Well strips)
Prlr Lyophilized Standard2 x 600 pg
100X Biotinylated Prlr Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)107103-110
EDTA Plasma (n=4)8580-90
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Prlr.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Prlr.
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Tips Information:

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