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PRKACA Protein (OPPA01114)

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1ug
$100.00
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Gene Symbol:
PRKACA
NCBI Gene Id:
5566
Alias Symbols:
cAMP-dependent protein kinase alpha-catalytic subunit, EC 2.7.11.11, PKA C-alpha, PKACA, PRKACA, MGC48865, MGC102831, PRKACA, cAMP-Dependent Protein Kinase A catalytic subunit alpha Recombinant
Description of Target:
Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha
Swissprot Id:
P17612
Protein Accession #:
NP_002721.1
Host:
E. Coli
Protein Name:
cAMP-dependent protein kinase catalytic subunit alpha
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PRKACA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PRKACA.
Product Format:
PKA catalytic subunit a is supplied in a buffer containing 25mM potassium phosphate (pH 6.5), 5mM 2-mercaptoethanol, 5mM EDTA, 150mM NaCI and 50% glycerol.
Purity:
Greater than 95% as determined by SDS-PAGE.
Additional Information:
Unit Definition: One unit is defined as the amount of recombinant PKA catalytic subunit a, required to incorporate 1umol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30C.
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Specific Activity: The specific activity of the recombinant PKA catalytic subunit a, is >15.000.000 U/mg.
Assay Conditions: Roskoski-AssayProtein kinase activity can be measured using a modified radioactive assay according to Roskoski et al.
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Product Description: cAMP-dependent PKA is an ubiquitous serine/threonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle, heart). The intracellular cAMP level regulates cellular responses by altering the interaction between the catalytic C and regulatory R subunits of PKA. The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits. Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. Different isoforms of catalytic and regulatory subunits suggest specific functions. The recombinant PKA catalytic subunit a is a 41kDa protein. The a-isoform is the predominant form with a broad tissue distribution and can be used for in vitro enzymological studies of neural and hormonal signal transduction or to phosphorylate target proteins in vivo including Ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism.
Reconstitution and Storage:
Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
Datasheets / Downloads:
OPPA01114
Protocol:
Tips Information:
 
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