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Prkaca ELISA Kit (Mouse) (OKEH04737)

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96 Wells
$425.00
In Stock
Gene Symbol:
PRKACA
Official Gene Full Name:
Protein kinase, cAMP dependent, catalytic, alpha
NCBI Gene Id:
18747
Alias Symbols:
C alpha, cAMP-dependent protein kinase catalytic subunit alpha, Cs, PKA, Pkaca, PKA C-alpha, PKCD
Description of Target:
This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome.
Protein Name:
CAMP-dependent protein kinase catalytic subunit alpha
Swissprot Id:
P05132
Protein Accession #:
NP_001264827.1
Nucleotide Accession #:
NM_001277898.1
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity:
< 0.15 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology Prkaca ELISA Kit (Mouse) (OKEH04737) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prkaca has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prkaca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prkaca captured in well.
Kit Component:
ComponentAmount
Prkaca Microplate96 Wells (12 x 8 Well Strips)
Prkaca Standard2
Sample Diluent1 x 20 mL
Prkaca Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PRKACA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PRKACA.
Datasheets / Downloads:
Printable datasheet for OKEH04737
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural mouse PKA C-alpha. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
41 kDa
Protein Size (# AA):
351
Protocol:
Tips Information:
 
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