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PPL ELISA Kit (Mouse) (OKCD00775)

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96 Wells
$555.00
In Stock
Application:
ELISA
Gene Symbol:
PPL
Official Gene Full Name:
periplakin
NCBI Gene Id:
19041
Alias Symbols:
AW553870, Periplakin
Description of Target:
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Protein Name:
periplakin
Swissprot Id:
Q9R269
Protein Accession #:
NP_032935
Nucleotide Accession #:
NM_008909.2
Species Reactivity:
Mouse
Sample Type:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity:
< 14.0 pg/mL
Kit Range:
31.25-2,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Ppl were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Ppl were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology Ppl ELISA Kit (Mouse) (OKCD00775) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppl has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppl is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Pplcaptured in well.
Kit Component:
ComponentAmount
Anti-Ppl Microplate96 Wells (12 x 8 Well Strips)
Ppl Lyophilized Standard2 x 2 ng
100X Biotinylated Ppl Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)79-101%78-89%83-95%98-105%
EDTA plasma(n=5)87-94%88-95%79-99%84-93%
heparin plasma(n=5)93-103%94-102%78-89%97-104%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)82-9986
EDTA plasma(n=5)78-10292
heparin plasma(n=5)96-105101
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PPL.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PPL.
Datasheets / Downloads:
Printable datasheet for OKCD00775
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Periplakin (PPL). No significant cross-reactivity or interference between Periplakin (PPL) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
1755
Protocol:
Tips Information:
 
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