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POLR2A Antibody (Phospho-Ser1619) (OAAF00054)

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100 ug
$289.00
In Stock

Conjugation Options

OAAF00054-FITC Conjugated

OAAF00054-HRP Conjugated

OAAF00054-Biotin Conjugated

Gene Symbol:
POLR2A
NCBI Gene Id:
5430
Official Gene Full Name:
DNA-directed RNA polymerase II subunit RPB1
Protein Name:
DNA-directed RNA polymerase II subunit RPB1
Swissprot Id:
P24928
Protein Accession #:
NP_000928.1
Alias Symbols:
POLR2,
Replacement Item:
This antibody may replace item sc-17798 from Santa Cruz Biotechnology.
Host:
Rabbit
Clonality:
Polyclonal
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Application:
WB, IHC, IF, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express POLR2A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express POLR2A.
Immunogen:
The antiserum was produced against synthesized peptide derived between 1585-1634 amino acids from the C-Terminal region of human POLR2A around the phosphorylation site of Ser1619.
Species Reactivity:
Human, Mouse, Rat
Product Format:
Liquid
Reconstitution and Storage:
Store at -20C/1 year
Datasheets / Downloads:
OAAF00054
Specificity:
POLR2A (phospho-Ser1619) antibody detects endogenous levels of POLR2A only when phosphorylated at serine 1619.
Formulation:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution:
The optimal working dilution should be determined by the end user.
Application Info:
WB: 1: 500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
Target Reference:
Tracy Jo Pasieka, J. Virol., Aug 2006; 80: 7600 - 7612.
Olaf R. Mook, Mol. Cancer Ther., Mar 2007; 6: 833 - 843.
Colleen A. McMullen, J. Neurosci., Jan 2004; 24: 161.
Protocol:
Tips Information:

Product Protocol: POLR2A antibody tested with COS7 in western blot (WB) (OAAF00054)

Please go here to see the protocol used for Aviva's POLR2A antibody (OAAF00054)

Product datasheet can be found here: POLR2A antibody (OAAF00054)

WB information:
Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using POLR2A (phospho-Ser1619) antibody. The lane on the right is treated with the synthesized peptide.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's POLR2A antibody (OAAF00054) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: POLR2A antibody tested with human breast carcinoma in immunohistochemistry (IHC) (OAAF00054)

Please go here to see the protocol used for Aviva's POLR2A antibody (OAAF00054)

Product datasheet can be found here: POLR2A antibody (OAAF00054)

IHC information:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using POLR2A (phospho-Ser1619) antibody. The picture on the right is treated with the synthesized peptide.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's POLR2A antibody (OAAF00054) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: POLR2A antibody tested with HeLa in immunofluorescence (IF) (OAAF00054)

Please go here to see the protocol used for Aviva's POLR2A antibody (OAAF00054)

Product datasheet can be found here: POLR2A antibody (OAAF00054)

IF information:
Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (phospho-Ser1619) antibody. The picture on the right is treated with the synthesized peptide.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's POLR2A antibody (OAAF00054) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

 
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