Aviva Systems Biology office will be closed for Thanksgiving - November 23rd, 2017 and November 24th, 2017.
Please go here for more info.

Now Offering Over 102,157 Antibodies & 44,722 Antigens!

PCDHGA3 ELISA Kit (Human) (OKCA01467)

Scroll Horizontally to view all Images
Print Page
  • Let us Help: Ask a Question
96 Wells
$600.00
In Stock
Gene Symbol:
PCDHGA3
Official Gene Full Name:
protocadherin gamma subfamily A, 3
NCBI Gene Id:
56112
Alias Symbols:
PCDH-GAMMA-A3
Description of Target:
Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
Protein Name:
Protocadherin gamma-A3
Swissprot Id:
Q9Y5H0
Protein Accession #:
NP_061739.2
Nucleotide Accession #:
NM_018916.3
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates
Sensitivity:
15.6 pg/mL
Kit Range:
62.5 - 4,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01467 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01467 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology PCDHGA3 ELISA Kit (Human) (OKCA01467) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PCDHGA3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PCDHGA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PCDHGA3 captured in the well.
Kit Component:
ComponentAmount
PCDHGA3 Microplate96 Wells (12 x 8 Well strips)
PCDHGA3 Lyophilized Standard2 x 4 ng
100X Biotinylated PCDHGA3 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat8882-95
1:29894-102
1:49790-103
1:88682-92
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9087-96
EDTA Plasma (n=4)10096-105
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PCDHGA3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PCDHGA3.
Datasheets / Downloads:
Printable datasheet for OKCA01467
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
X
Ask a Question

Tell us what you think about this item!

Write A Review
    Please, wait...